Thermo Fisher Scientific DAP3 Monoclonal Antibody (OTI10B10), TrueMAB
DAP3 단백질을 인식하는 Mouse monoclonal antibody로 Western blot에 적합. Human 시료 반응성. 동결건조 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10B10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 161–398 of human DAP3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and function in mitochondrial protein synthesis. Mitoribosomes consist of 28S and 39S subunits, with a higher protein-to-rRNA ratio than prokaryotic ribosomes. Unlike prokaryotic ribosomes, mammalian mitoribosomes lack a 5S rRNA. The DAP3 gene encodes a 28S subunit protein involved in apoptotic pathways initiated by TNF-α, Fas ligand, and IFN-γ. It may bind ATP/GTP and interact with mitoribosomal protein S27. Splice variants exist but encode the same protein. Pseudogenes are located on chromosomes 1q and 2q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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