Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 647
Alexa Fluor 647로 결합된 염소 항-닭 IgY (H+L) 2차 항체로, WB, IHC, ICC/IF 등 다양한 형광 이미징에 적합합니다. 높은 형광 안정성과 감도를 제공하며, 650/671 nm 파장에서 최적의 신호를 발현합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- A21449
- 판매단위
- pk
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Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 647
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 647 |
| Excitation/Emission Max | 650/671 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2535866 |
| Target | IgY |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- AP
- Biotin
- FITC
- HRP
- Texas Red
- TRITC
- Request custom conjugation available
Product Specific Information
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye ideally suited to the 647 nm laser line. It provides stable signal generation for imaging and flow cytometry and is pH-insensitive over a wide molar range.
This dye offers high fluorescence quantum yield and photostability, enabling sensitive detection of low-abundance biological structures. Alexa Fluor 647 molecules can be attached to proteins at high molar ratios without significant self-quenching, resulting in brighter conjugates and more sensitive detection.
The degree of labeling for each conjugate is typically 2–8 fluorophore molecules per IgG molecule; the exact labeling ratio is indicated on the certificate of analysis for each lot.
Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to remove aggregates that may cause nonspecific background staining. Optimal antibody dilution should be determined empirically; for most applications, 1–10 µg/mL is suitable.
Product is shipped at room temperature.
Target Information
Anti-Chicken secondary antibodies are affinity-purified with well-characterized specificity for chicken immunoglobulins. They are useful for detection, sorting, or purification of specific targets.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and are further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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