Thermo Fisher Scientific DNAJA2 Monoclonal Antibody (OTI2A2), TrueMAB
DNAJA2 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. HEK293T 세포에서 생산된 full-length human recombinant protein을 면역원으로 함. 연구용으로만 사용되며, 고순도 친화 크로마토그래피 정제 및 안정적인 동결건조 형태 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A2 |
| Immunogen | Full-length human recombinant protein of human DNAJA2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The DNAJA2 protein belongs to the evolutionarily conserved DNAJ/HSP40 family, which regulates molecular chaperone activity by stimulating ATPase activity.
DNAJ proteins typically contain three domains:
- A conserved 70-amino acid J domain at the N-terminus
- A glycine/phenylalanine (G/F)-rich region
- A cysteine-rich domain with four motifs resembling a zinc finger domain
DNAJA2 functions as a cochaperone of Hsp70s, aiding in protein folding and mitochondrial protein import in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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