Thermo Fisher Scientific Phospho-beta Catenin (Tyr333) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phosphorylated beta-catenin (Tyr333). Validated for WB and IHC(P). Supplied as liquid, 1 mg/mL, stored at −20 °C. Useful for studying cell adhesion and Wnt signaling pathways in cancer research.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 333 (Y-T-Y(p)-E-K) derived from Human Beta-catenin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | −20 °C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554155 |
Product Specific Information
A suggested positive control for Western blot is 293 cells; suggested positive control for IHC is human lung carcinoma.
Target Information
Beta-catenin, an adherens junction (AJ) protein, is a key component of cell–cell adhesion structures. AJs are essential for epithelial layer formation and maintenance by regulating cell growth and adhesion. Beta-catenin interacts with the cytoplasmic domain of E-cadherin, linking it to alpha-catenin and anchoring the complex to the actin cytoskeleton.
It also associates with cytoskeletal complexes containing adenomatous polyposis coli protein and microtubules, participating in signaling pathways involving tyrosine kinases, phosphatases, and Wnt/Wingless. These interactions regulate morphogenesis and cell proliferation.
Beta-catenin is expressed in hair follicle cells, basal and peripheral matrix cells, and inner/outer root sheath cells. Aberrant beta-catenin activity has been implicated in pilomatrixoma, medulloblastoma, colorectal, ovarian, and other cancers. In the nucleus, it coactivates Lef/Tcf transcription factors that drive expression of genes such as cyclin D and c-myc, promoting tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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