Thermo Fisher Scientific GeneChip Human Gene 2.0 ST Array, 6 arrays

Applied Biosystems™ GeneChip™ Human Gene 2.0 ST Array

The GeneChip™ Human Gene 2.0 ST Array provides the most accurate, sensitive, and comprehensive measurement of protein-coding and long intergenic non-coding RNA (lincRNA) transcripts.

Comprehensive Design

Keeping pace with the research community’s understanding of the transcriptome, these whole-transcript arrays include probes to measure both messenger RNA (mRNA) and long intergenic non-coding RNA transcripts (lincRNA).
This design enables a complete expression profile of mRNA as well as intermediary lincRNA transcripts that impact mRNA expression.

Research over the past 20 years has predominantly focused on protein-coding mRNA transcripts and their role in cellular processes such as disease and development. Recently, more than 10,000 transcripts (>200 bases) with little or no protein-coding potential have been identified.
Although only a small fraction of these non-coding RNAs have functional annotations, differential expression of lincRNAs is known to play an important role in disease genesis and progression, including cancer.

Recent advancements in transcriptome profiling have shown the association of lincRNAs in various cellular functions:

• Regulation of mRNA transcription
• Regulation of mRNA post-transcriptional modifications
• Occlusion/recruitment of transcription factor binding
• Activation and transportation of transcription factors
• Interaction with accessory proteins
• Guiding protein complexes to locations in the genome

Key Benefits

• Comprehensive coverage for discovering novel biological insights

• 30,000 coding transcripts

• 11,000 long intergenic non-coding transcripts

• Highly reproducible (Intra-lot correlation coefficient = 0.99)

Content Profile

Since the design of the Human Gene 1.0 ST Array, numerous new lincRNAs have been identified.
The Human Gene 2.0 ST Array was developed to measure differential expression of this class of RNA transcripts.
To supplement RefSeq lincRNA data, sequences and transcripts were sourced from:

• lncRNA db
• Broad Institute Human Body Map lincRNAs and TUCP (Transcripts of Uncertain Coding Potential) catalog

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