Corning® BioCoat™ Collagen IV-coated Flasks

Type IV Collagen is a ubiquitous component in basement membranes and provides the major structural support for this matrix. When the Collagen IV meshwork is assembled, it provides a scaffold for the assembly of other basement membrane components through interactions with laminin, entactin/nidogen, and heparan sulfate proteoglycan. Collagen IV is useful as a substrate for growth of epithelial, endothelial, muscle, and nerve cells. Collagen plays a role in the regulation of cell growth, differentiation and adhesion, as well as tissue formation.

Applications include:

• Promotion of cell attachment and spreading
• Cell differentiation and neurite outgrowth
• Increased proliferation of PC12 cells
• Studies of effects of collagen IV on cell behavior
• Cell adhesion assays

Source

• Engelbreth-Holm-Swarm (EHS) lathrytic mouse tumor

Quality Control

• Tested for ability to initiate differentiation (neurite outgrowth) of NG-108 rat glioma/mouse neuroblastoma cells
• Tested and found negative for bacteria and fungi
• Collagen IV purity >90% by SDS-PAGE

Storage

• 2°C to 8°C. Do not freeze.

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