Thermo Fisher Scientific F1A alpha Polyclonal Antibody
Rabbit polyclonal antibody recognizing human F1A alpha protein. Validated for WB, IHC, and ICC/IF applications. Supplied as liquid, 0.9 mg/mL concentration. Suitable for apoptosis and death receptor pathway research.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human F1A alpha (UniProt ID: Q9UK73-1, amino acids 262–349) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2641186 |
Product Specific Information
Immunogen sequence:
NDRENYDIIK TYHYLYLAML ERFQDGDNIL EKEVLPPIHA YGNRTECRNP QELESIRQDR DALHMEGLIV RERILGADNI DVSHPIIY
Sequence identity to orthologs:
- Mouse: 98%
- Rat: 98%
Target Information
Fas and tumor necrosis factor receptor 1 (TNFR1) are prototype members of the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was identified as F1Aalpha (also known as FEM1-b). F1Aalpha/FEM1-b is homologous to the C. elegans sex-determining protein FEM-1. It is cleaved by CED-3 and caspase, and associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis, making it a key member of the death receptor-associated proteins mediating apoptosis. F1Aalpha is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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