Thermo Fisher Scientific AUH Polyclonal Antibody
Thermo Fisher Scientific의 AUH Polyclonal Antibody는 인간 AUH 단백질을 인식하는 토끼 IgG 항체로, Western blot과 IHC(P)에서 사용 가능합니다. 합성 펩타이드 기반 항원으로 제작되었으며, 고순도 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4–8 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the C-terminal of human AUH (aa 228–277) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2607896 |
Product Specific Information
Peptide sequence:
IGMSLAKELI FSARVLDGKE AKAVGLISHV LEQNQEGDAA YRKALDLARE
Sequence homology:
Cow: 100%
Dog: 100%
Guinea Pig: 93%
Horse: 100%
Human: 100%
Mouse: 100%
Rabbit: 100%
Rat: 100%
Yeast: 75%
Target Information
The methylglutaconyl-CoA hydratase, mitochondrial protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation.
AUH is homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity.
A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog, which shares 92% identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial AU-binding protein.
Human AUH is expressed as a single mRNA species of 1.8 kb and translated as a 40 kDa precursor protein, subsequently processed to a 32 kDa mature form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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