Thermo Fisher Scientific BARD1 Polyclonal Antibody
BARD1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. KLH 결합 합성 펩타이드(aa 101-200)를 면역원으로 사용하며, 고순도 Protein A 정제 및 안정적인 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human BARD1, amino acids 101–200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The BARD1 gene encodes a protein that interacts with the N-terminal region of BRCA1. It shares homology with the two most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in various proteins regulating cell growth, including tumor suppressors and proto-oncogenes. BARD1 also contains three tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, suggesting that a stable complex between these proteins is essential for BRCA1 tumor suppression. BARD1 may be a target of oncogenic mutations in breast or ovarian cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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