Thermo Fisher Scientific Calpain 7 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-220 of human CAPN7 (NP_0551111) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calpain 7,uniProtId:Q9Y6W3-1,ncbiNodeId:9606,antigenRange:20-220,antigenLength:813,antigenImageFileName:PA5-116762_Calpain_7_Q9Y6W3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116762_Calpain_7_Q9Y6W3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.36 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901393

Product Specific Information

Positive Samples: Jurkat

Immunogen sequence: RDHEGRYSEA VFYYKEAAQA LIYAEMAGSS LENIQEKITE YLERVQALHS AVQSKSADPL KSKHQLDLER AHFLVTQAFD EDEKENVEDA IELYTEAVDL CLKTSYETAD KVLQNKLKQL ARQALDRAEA LSEPLTKPVG KISSTSVKPK PPPVRAHFPL GANPFLERPQ SFISPQSCDA QGQRYTAEEI EVLRTTSKIN G

Target Information

Calpain proteinases are defined currently as papain-like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain-4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date (calpain 5, 6, 8, 9, 10, 11, 12, 13, 14, 15) are not known to require a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain-T in calpains 5 & 6, domain-N for calpain-7). Calpain-7 domain-N does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. In place of the EF hand calcium-binding domain IV, calpain-7 has a sequence most closely resembling domain-III, with unknown function. Calpain-7 is widely distributed, found in most tissues analyzed.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.