Thermo Fisher Scientific CD34 Monoclonal Antibody (MEC14.7), Biotin
CD34 단백질을 인식하는 rat monoclonal antibody(MEC14.7)로, Biotin으로 conjugation됨. Flow cytometry, IHC, ELISA 등 다양한 응용 가능. Stem cell marker 연구용으로 적합하며, 고순도 Protein G 정제 및 안정적 PBS/BSA buffer 제공.
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Thermo Fisher Scientific CD34 Monoclonal Antibody (MEC14.7), Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Flow Cytometry (Flow) | 0.5 µg/1×10^6 cells |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEC14.7 |
| Immunogen | Mouse CD34 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4–5 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539211 |
Additional Formats
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on various stem cell populations. It functions as a stem cell marker and may act as a surface receptor involved in endocytosis, adhesion, differentiation, and proliferation of hematopoietic stem cells. CD34 expression represents specific stages of hematopoietic development and may mediate attachment of stem cells to the bone marrow extracellular matrix or stromal cells. It could also serve as a scaffold for lineage-specific glycans, facilitating binding to lectins in the marrow environment. The intracellular domain is phosphorylated by activated protein kinase C, indicating a potential role in signal transduction. CD34 dysfunction has been associated with dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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