
Thermo Fisher Scientific Aspartate Transaminase Polyclonal Antibody
돼지 심장에서 유래한 Aspartate Transaminase(AST)를 인식하는 Sheep Polyclonal Antibody. WB, ELISA, IP에 사용 가능하며, 4°C 단기 보관 및 -20°C 장기 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Pig |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Aspartate Aminotransferase (AST) [Pig Heart] |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 96 mg/mL |
| Storage Buffer | 0.02M potassium phosphate / whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
Cross-reactivity against AST from other tissues and species may occur but has not been specifically determined.
Target Information
GOT1 (Glutamic-oxaloacetic transaminase) is a pyridoxal phosphate-dependent enzyme that exists in cytoplasmic (GOT1) and mitochondrial (GOT2) forms.
It plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles.
Both enzymes are homodimeric and show close homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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