
Thermo Fisher Scientific Peroxidase Polyclonal Antibody
Horseradish Peroxidase(HRP)에 특이적인 Rat Polyclonal Antibody로 Western blot, IHC, ELISA, IP 등 다양한 면역분석에 사용 가능. 고농도(85 mg/mL) 동결건조 형태로 제공되며, 높은 안정성과 재현성을 가짐. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:2,000–1:6,000 |
| Immunoprecipitation (IP) | 1:200–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peroxidase (Horseradish) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 85 mg/mL |
| Storage Buffer | Whole serum / PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rat serum, purified and partially purified Peroxidase (Horseradish).
Cross-reactivity against peroxidase from other tissues and species may occur but has not been specifically determined.
Target Information
Horseradish Peroxidase (HRP) is an enzyme derived from the root of the horseradish plant. It catalyzes the oxidation of a wide range of substrates in the presence of hydrogen peroxide. HRP is widely used as a detection enzyme in immunoassays such as ELISA and Western blot due to its high sensitivity and stability. It is also used as a tracer in enzyme-linked assays and as a labeling agent in fluorescent microscopy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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