Thermo Fisher Scientific SMCR8 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SMCR8. Recombinant protein control fragment (Product #RP-93456). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMCR8,uniProtId:Q8TEV9-1,ncbiNodeId:9606,antigenRange:279-396,antigenLength:937,antigenImageFileName:PA5-54449_SMCR8_Q8TEV9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-54449_SMCR8_Q8TEV9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647630

Product Specific Information

Immunogen sequence: SQASTTSNPD ESADTDLYTC RPAYTPKLIK AKSTKCFDKK LKTLEELCDT EYFTQTLAQL SHIEHMFRGD LCYLLTSQID RALLKQQHIT NFLFEDFVEV DDRMVEKQES IPSKPSQD

Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 86%.

Target Information

Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity. Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates. In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.