Thermo Fisher Scientific Human EGF Recombinant Protein

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Not Applicable

Expression System

E. coli

Amino acid sequence

Human EGF recombinant protein contains 53 amino acids

Molecular weight

6.2 kDa

Class

Recombinant

Type

Protein

Activity

1x10^7 Units/mg compared to reference standards

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1 mg/mL

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

This is a recombinant protein suitable for use as a control in ELISA and Western blot applications.

Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20ºC. Upon reconstitution, store at 4ºC for 2-7 days or aliquot and store at -20ºC. For long-term storage add either HSA or BSAQ to 0.1%. Avoid repeated freeze-thaw cycles.

Reconstitute using distilled water to a concentration not less than 100 µg/mL.

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.