Thermo Fisher Scientific Human EGF Recombinant Protein

Applications

• Control (Assay-dependent)
• Suitable for ELISA and Western blot applications

Publications

• Miscellaneous PubMed: 1 publication available

Product Specifications

Product Specific Information

This recombinant protein is suitable for use as a control in ELISA and Western blot assays.
Lyophilized product is stable at room temperature for up to 3 weeks. Store lyophilized product desiccated at -20°C.
Upon reconstitution, store at 4°C for 2–7 days or aliquot and store at -20°C.
For long-term storage, add HSA or BSA to a final concentration of 0.1%. Avoid repeated freeze-thaw cycles.
Reconstitute using distilled water to a concentration not less than 100 µg/mL.

Target Information

Epidermal Growth Factor (EGF) binds to the EGF Receptor (EGFR), a 170 kDa protein kinase. Activation of EGFR initiates multiple cellular pathways in response to environmental stimuli.
EGFR forms homo- or heterodimers with other family members, each initiating distinct signaling pathways via SH2-containing effector proteins.
EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins such as GRB2, activating downstream cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT pathways.
Research suggests EGF may also be involved in NF-κB signaling activation.
Defects in the EGF gene are linked to hypomagnesemia type 4, and dysregulation has been associated with cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.