Thermo Fisher Scientific FOXO4 Monoclonal Antibody (OTI5A5), TrueMAB
FOXO4 단백질을 인식하는 단클론 항체로, Western blot에 적합합니다. Human, Mouse, Rat 시료에 반응하며, E.coli에서 생산된 인간 FOXO4 단백질 조각(aa 206-505)을 면역원으로 사용했습니다. 동결건조 형태로 공급되며, PBS(pH 7.3, 8% trehalose) 완충용액에 보관합니다.
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Applications
- Western Blot (WB): 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5A5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 206-505 of human FOXO4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. Also known as AFX1 or MLLT7, FOXO4 is a member of the O class of forkhead/winged helix transcription factors, along with FOXO1, FOXO3, and FOXO6.
It is highly expressed in skeletal muscle and adipose tissue, binds to the Insulin Responsive Element (IRE), and activates downstream targets of the Insulin/IGF1 signaling pathway such as IGFBP1.
Phosphorylation by Akt leads to FOXO4 binding to 14-3-3 proteins and subsequent nuclear export.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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