Thermo Scientific™

GeneJET Endo-Free Plasmid Maxiprep Kit

Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit is a fast, convenient, and efficient system for the large scale purification of자세히 알아보기

Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit is a fast, convenient, and efficient system for the large scale purification of endotoxin-free plasmid DNA. Endotoxins are major contaminants found in plasmid DNA preparations. For transfection of sensitive cell lines endotoxins are a major problem. The same applies to in vivo transfection as endotoxins can elicit strong immune responses. Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep Kit is designed to reduce endotoxin level to <0.1 EU/μg of plasmid DNA. The kit utilizes a silica-based membrane technology in the form of convenient spin columns.

Each preparation recovers up to 1000 μg of high copy plasmid DNA from 350 mL of recombinant E. coli cultures that can be used in a wide variety of molecular biology procedures such as transfection of sensitive cells (e.g. Huh-7), in vivo transfection, transformation, restriction endonuclease digestion, automated sequencing, and in vitro transcription.

Highlights

• Pure—free of endotoxins plasmid DNA suitable for transfection of sensitive cells
• Efficient—plasmid recovery resulting in high DNA yields
• Fast—isolation of high quality plasmid DNA in less than 60 minutes
• Convenient—spin column format, centrifuge or vacuum protocol

Applications

• Transfection of sensitive cell lines
• in vivo transfection
• Automated sequencing
• PCR
• Transformation
• in vitro transcription

Includes
• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Endotoxin Binding Reagent
• Wash Solution I (conc.)
• Wash Solution II (conc.)
• RNase A Solution
• Elution Buffer
• GeneJET Endo-Free Maxi Purification Columns & Collection Tubes (50 mL, blue-capped)
• GeneJET Endo-Free Maxi Filtration Columns & Collection Tubes (50 mL, white-capped)

사양

농도400 ng/μL

컬럼 유형Spin Column

형식Spin Column

Isolation TechnologySilica Column

스케일Maxi

샘플 종류Bacteria

용도(애플리케이션)Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR

고처리량 호환성Not High-throughput Compatible (Manual)

반응 수10 Preps

프렙 스케일> 200 μg (Large-Scale) Plasmid DNA

제품라인GeneJET

제품 유형Endo-Free Plasmid MaxiPrep Kit

수량10 Preps

타겟Plasmid DNA

테스트 시간60 min.

Unit SizeEach