Thermo Fisher Scientific TCF12 Polyclonal Antibody
TCF12 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, IP 등에 사용 가능. Human에 반응하며 Mouse와 Rat에도 예측 반응. 항원 친화 크로마토그래피로 정제된 액상 제품으로, 4°C 보관 및 Wet ice 배송.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of Human Transcription Factor 12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
TCF12 (also known as HTF4) is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG.
It is expressed in various tissues including skeletal muscle, thymus, B- and T-cells, and participates in regulating lineage-specific gene expression through heterodimer formation with other bHLH E-proteins.
TCF12, together with E2A, is essential for blocking thymocyte proliferation before pre-TCR expression and is critical for T cell differentiation.
It is also a key factor in the development of invariant natural killer T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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