
Thermo Fisher Scientific Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, HRP
HRP 결합형 염소 항-마우스 IgG Fc 폴리클로날 2차 항체로, WB, IHC, ELISA 등 다양한 면역실험에 적합. 교차 흡수 처리로 비특이적 반응 최소화. 고순도 친화 크로마토그래피 정제. 안정적 장기 보관 가능.
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Thermo Fisher Scientific Goat anti-Mouse IgG Fc Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term; for long term store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2534764 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, and human serum proteins |
| Antibody Form | Whole Antibody |
Additional Formats Available
- AP
- APC
- Biotin
- FITC
- PE
- TRITC
- Custom conjugation available upon request
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand for 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to:
- Non-immunoglobulin mouse serum proteins
- Light chains on all mouse immunoglobulins
- Mouse IgG F(ab')₂ fragment
- Serum proteins from bovine, horse, or human
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of specific targets. Secondary antibodies offer versatility, allowing use with various detection systems (e.g., HRP, AP, fluorescence) and provide enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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