Thermo Fisher Scientific ADFP Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 사용 가능한 ADFP 폴리클로날 항체. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고순도 제품. PBS와 글리세롤 기반 용액 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full length Human ADFP (aa 1–437, UniProt ID: Q99541-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803955 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Milk lipid globules from humans, cows, and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase.
ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial, and epithelial cells. In tissues, expression of adipophilin is restricted to specific cell types such as:
- Lactating mammary epithelial cells
- Adrenal cortex cells
- Sertoli and Leydig cells of the male reproductive system
- Steatosis or fatty change hepatocytes in alcoholic liver cirrhosis
ADFP may be a marker for identifying specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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