Thermo Fisher Scientific HLA-G (Major Histocompatibility Complex, class I, G) Monoclonal Antibody (HLAG, 6686)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed):

• Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin).
The heavy chain is anchored in the membrane and is approximately 45 kDa. The gene contains 8 exons:

• Exon 1: leader peptide
• Exons 2–3: alpha1 and alpha2 domains (peptide-binding)
• Exon 4: alpha3 domain
• Exon 5: transmembrane region
• Exon 6: cytoplasmic tail

HLA-G is expressed on fetal-derived placental cells.
HLA and MHC antibodies play a significant role in immunopeptidomics, aiding in neoantigen identification and characterization via high-performance liquid chromatography coupled with tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.