Thermo Fisher Scientific Alkaline Phosphatase Polyclonal Antibody, HRP

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
Cross-reactivity against Alkaline Phosphatase from other tissues and species may occur but has not been specifically determined.

Target Information

Alkaline phosphatase (ALP) is a homodimeric enzyme (86 kDa) containing two zinc atoms crucial for its catalytic function per monomer.
It is optimally active at alkaline pH and found in both prokaryotes and eukaryotes.
ALP plays a role in liver metabolism and skeletal development.
In bacteria, ALP acts as a source of inorganic phosphate, and in yeast, it serves as a model for studying membrane protein sorting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.