Invitrogen™

Vybrant™ DyeCycle™ Orange Stain

Vybrant™ DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488자세히 알아보기

Vybrant™ DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488 nm or 532 nm laser lines.

• Precise—accurate cell cycle analysis in living cells

• Safe—low cytotoxicity for cell sorting and additional live cell experiments

• Simple, robust staining protocol

View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

Precise

Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

Simple, Robust Staining Protocol

For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2 μL of Vybrant™ DyeCycle™ Orange Stain. Final stain concentration is 10 μM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on flow cytometer using 488 nm excitation and orange emission.

사양

농도5 mM

검출 방법Fluorescence

용도 (장비)Flow Cytometer

제품 유형Stain

염료 유형Vybrant™ DyeCycle™ Orange

방출532, 488

형태Solution

형식Tube(s)

제품라인DyeCycle™

수량400 μL

배송 조건Room Temperature

용해도Water

세포하위구조 국지화Nucleic Acids

Unit Size400 µL