Thermo Fisher Scientific NKIRAS1 Monoclonal Antibody (OTI8B11), TrueMAB
Human NKIRAS1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF에 사용 가능. Lyophilized 형태이며, 1 mg/mL 농도로 제공. 연구용으로 NF-kappa-B 관련 신호전달 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8B11 |
| Immunogen | Full length human recombinant protein of human NKIRAS1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
KappaB ras-1 (kappa-B-ras-1) and kappaB-ras-2 are small Ras-like GTPases that associate with Ikappa-B, an inhibitor of NF-kappa-B.
Ikappa-B-alpha and Ikappa-B-beta are homologous forms; Ikappa-B-beta contains a unique 47-amino acid region within its ankyrin domain.
Inactive Ikappa-B-alpha–NF-kappa-B complexes shuttle between nucleus and cytoplasm, whereas Ikappa-B-beta–NF-kappa-B complexes remain cytoplasmic.
KappaB-ras proteins preferentially bind Ikappa-B-beta through this unique insert, modulating its localization and degradation rate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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