Thermo Fisher Scientific 4EBP2 Polyclonal Antibody
4EBP2 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체. 인간, 마우스, 랫트 반응성. IHC, ICC/IF, ELISA 등 다양한 응용 가능. Protein A로 정제된 액상 형태로, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human 4E-BP2 (amino acids 21–120) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
PHAS-II (eIF4E-binding protein II) is involved in translation initiation. PHAS-II expression is highest in liver and kidney. PHAS proteins bind to eIF-4E, the mRNA cap-binding protein, and inhibit translation of capped mRNA both in vitro and in cells. Phosphorylation at appropriate sites promotes dissociation of PHAS/eIF-4E complexes, allowing eIF-4E to bind eIF-4G (p220), thereby increasing formation of the eIF-4F complex and enhancing translation initiation. Evidence suggests that the mTOR/p70S6K pathway regulates PHAS-I and -II, indicating that PHAS proteins mediate effects of this pathway on protein synthesis and cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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