Thermo Fisher Scientific Phospho-Arhgap22 (Ser22) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (IHC)

1:100-1:500

-

ELISA (ELISA)

1:20,000-1:60,000

-

Immunoprecipitation (IP)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ARHGAP22,uniProtId:Q8BL80-1,ncbiNodeId:10090,antigenRange:22,antigenLength:702,antigenImageFileName:600-401-J11_ARHGAP22_Q8BL80-1_Rabbit.svg,antigenImageFileNamePDP:600-401-J11_ARHGAP22_Q8BL80-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.07 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-ARHGAP22 pS22 is specific for phosphorylated ARHGAP22 at Serine 22. It also recognizes the S397->A mutation but not the S22->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS22 from other sources has not been determined.

Target Information

GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in down regulation of their active form. ARHGAP22 (Rho GTPase activating protein 22), also known as RHOGAP2, is a 698 amino acid protein that localizes to the cytoplasm, and contains one Rho-GAP domain and one PH domain. ARHGAP22 functions as a GTPase-activating protein for Rac 1 converting it to an inactive GDP-bound state and, through its interaction with ZNF161 (VEZF1), is thought to be useful in transcription regulation. Multiple isoforms of ARHGAP22 exist due to alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.