Applied Biosystems™

TaqMan™ Copy Number Assay

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA자세히 알아보기

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software. TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction; the copy number assay detects the target sequence, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. The results are then analyzed by the relative quantitation method using Applied Biosystems CopyCaller Software.

The predesigned TaqMan Copy Number Assay collection consists of human and mouse assay libraries for copy number variation (CNV) analysis. The human assay collection includes over 1.6 million assays targeting gene exons and introns, extragenic regions, and CNV sequences from the Database of Genomic Variants (DGV). The mouse collection includes over 180,000 assays targeting gene exons. Assays to common vector marker and reporter genes are also available for transgenic studies.

Benefits:
• Specific—gold-standard TaqMan chemistry with minor groove binder (MGB) probes increase assay specificity by enabling shorter probes
• Reproducible—robust assay designs developed with our bioinformatics pipeline enable accurate, reproducible, and reliable results
• Easy to interpret—CopyCaller Software provides the calculated copy number and predicted copy number, along with confidence value and z-score quality metrics
• Fast and simple—setup to primary analysis in 3–4 hours

Approximate ship time
4–6 days in North America and 6–10 days in Europe

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

TaqMan Copy Number Reference Assays are sold separately.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

사양

3`프라이머 수정None

5`프라이머 수정None

검출 방법Primer-probe

형태Frozen

PCR 방법qPCR

반응 속도Fast

용도(장비)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex

그린 기능Sustainable packaging

내부 프로브 수정FAM (5), MGB (Minor Groove Binder) (3)

라벨 또는 염료FAM

제품라인TaqMan™

제품 유형Copy Number Assay

정제 방법Solid Phase Extraction (SPE)

순도 또는 품질 등급RNase-Free, DNase-Free

수량S (360 reactions), made to order

배송 조건Room Temperature

종Human

농도20X

반응 수360 reactions

부피360 μL

Unit SizeEach