Thermo Fisher Scientific POU6F1 Polyclonal Antibody
Rabbit Polyclonal antibody against human POU6F1 for IHC and IHC (Paraffin) applications. Antigen affinity purified, supplied as liquid in PBS with 40% glycerol. Recognizes Brn-5 protein, expressed in developing brain and spinal cord. For research use o...
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 | View 1 publication |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human POU6F1. Recombinant protein control fragment (Product #RP-100775). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645793 |
Product Specific Information
Immunogen sequence:
LLLNAQGQVI ATLASSPLPP PVAVRKPSTP ESPAKSEVQP IQPTPTVPQP AVVIASPAPA AK
Highest antigen sequence identity to the following orthologs:
- Mouse: 85%
- Rat: 87%
Target Information
Protein: Brn-5 (Brain-5), also known as POU6F1, MPOU, or TCFB1
Brn-5 is widely expressed but during embryogenesis is exclusively found in the developing brain and spinal cord. It contains two DNA-binding domains (POU-specific domain and POU homeodomain), each with an HTH (helix-turn-helix) motif. Brn-5 binds to CRH (corticotrophin-releasing hormone) elements with high affinity and can enhance Prolactin gene expression and activate Pit-1 expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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