Thermo Fisher Scientific ASAH2 Polyclonal Antibody
ASAH2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC(P), ICC/IF에 적합. 인간 시료 반응성. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관. 세라마이드 대사 연구에 유용.
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- PA563928
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ASAH2. Recombinant protein control fragment (Product # RP-103744) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638264 |
Product Specific Information
Immunogen sequence:
RTFGDVLQPA KPEYRVGEVA EVIFVGANPK NSVQNQNHQT
Highest antigen sequence identity:
- Mouse: 85%
- Rat: 83%
Target Information
Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and a key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5–8.5.
ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis by reducing C16-ceramide and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.
ASAH2 is ubiquitously expressed, with higher levels in the intestine, kidney, skeletal muscle, and heart. Recent studies indicate that ASAH2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates bioactive sphingolipid metabolites in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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