Invitrogen™

PureLink™ Genomic Plant DNA Purification Kit

The PureLink™ Genomic Plant DNA Purification Kit allows efficient isolation of total DNA in about 40 minutes from a variety자세히 알아보기

The PureLink™ Genomic Plant DNA Purification Kit allows efficient isolation of total DNA in about 40 minutes from a variety of plant samples, such as spinach, tomato, soy, wheat, Arabidopsis, and mushroom.
• Fast – Total DNA purification from difficult plant samples in under an hour
• Efficient – Yields high-quality DNA with minimal RNA contamination

Simple DNA Purification from Plant Samples
The PureLink™ Genomic Plant DNA Purification Kit is suitable for isolating DNA from chloroplasts and a variety of plant tissues (Figure 1), including alfalfa sprouts, sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass, and Arabidopsis thaliana leaves, in less than 40 minutes. The entire protocol relies on the centrifugation of spin columns in the absence of organic solvents. PureLink™ Genomic Plant DNA Purification Kit is designed to purify DNA from up to 100 mg of plant tissue.

Maximize Yields of Total DNA
High yield of DNA and minimal DNA degradation is achieved by using young plant samples such as leaves and by freezing the sample in liquid nitrogen immediately after collection. Total DNA is eluted in 2 aliquots of 100 μl each to obtain greater DNA yield, with the first elution generating between 65-80% DNA recovery and the second elution > 90%. Typically, DNA isolated using the PureLink™ Genomic Plant DNA Purification Kit has an A260/A280 > 1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a Number of Downstream Applications:
Total DNA purification from different plant tissue with the PureLink™ Genomic Plant DNA Purification Kit is suitable for a variety of applications, including PCR, restriction enzyme digestion, and Southern blotting.

For Research Use Only. Not intended for diagnostic procedures.

사양

컬럼 유형Spin Column

Isolation TechnologySilica Spin Column

스케일Mini

샘플 종류Plants

용리량100 to 200 μL

최종 제품 유형gDNA

용도(애플리케이션)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization

고처리량 호환성Not High-throughput Compatible (Manual)

수량50 Preps

배송 조건Room Temperature

테스트 시간40 min.

수율Up to 14.6 μg

시작 물질 양100 mg

Unit SizeEach