Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 30 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Yellow series
• eFluor 450, FITC, PE, PE-Cyanine5, PE-eFluor 610
• PerCP-eFluor 710, Super Bright series
• NovaFluor Blue / Red / UV / Violet / Yellow series
• Request custom conjugation

Product Specific Information

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells, and is upregulated upon T-cell stimulation. CD27 binds to CD70 and plays an important role in T cell–B cell interaction.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells
  • Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume)
  • Cell count: 10⁵–10⁸ cells/test (empirically determined)

Light Sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Excitation/Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily, including CD40 and CD30. It regulates cell proliferation and death, with expression mainly confined to lymphocytes. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subpopulation of B cells.

Activation of T cells via the TCR-CD3 complex upregulates CD27 expression and releases its soluble form (sCD27, 28–32 kDa), detectable in plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker in acute and chronic B cell malignancies. CD27-binding protein (SIVA) is a proapoptotic protein involved in CD27-mediated apoptosis. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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