Thermo Fisher Scientific ATP6V1F Monoclonal Antibody (OTI1B8)
ATP6V1F 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC/IF, 유세포분석에 적합합니다. 인간 시료에 반응하며, 고순도 친화 크로마토그래피 정제 제품으로 안정적 보관이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B8 |
| Immunogen | Full length human recombinant protein of ATP6V1F produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723947 |
Target Information
ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase–dependent organelle acidification is essential for processes such as:
- Protein sorting
- Zymogen activation
- Receptor-mediated endocytosis
- Synaptic vesicle proton gradient generation
V-ATPase comprises:
- V1 domain: Three A and three B subunits, two G subunits, and C, D, E, F, H subunits (contains ATP catalytic site)
- V0 domain: Five different subunits (a, c, c’, c”, and d)
Multiple isoforms of V1 and V0 subunit proteins are encoded by different genes or alternative splicing variants.
This encoded protein corresponds to the V1 domain F subunit protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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