Thermo Fisher Scientific E2F1 Recombinant Rabbit Monoclonal Antibody (23GB1505)
E2F1 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. 인간, 마우스, 랫트 반응성을 가지며 HEK293F 발현 시스템에서 생산되었습니다. 고순도 친화 크로마토그래피 정제, 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB1505 |
| Immunogen | A synthesized peptide derived from human E2F1 (355–400AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins. It is also a target of transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains:
- DNA binding domain
- Dimerization domain (interaction with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain embedded within the transactivation domain
E2F1, along with E2F2 and E2F3, possesses an additional cyclin binding domain. E2F1 binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner and can mediate both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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