Thermo Fisher Scientific Caspase 3 Monoclonal Antibody (74T2)
Caspase 3 단백질을 인식하는 mouse monoclonal antibody(클론 74T2). Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 등 반응. Protein A 정제, 0.5 mg/mL 농도, PBS buffer(0.1% sodium azide) 보존.
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Applications 및 권장 희석 배수
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 7 publications |
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:10–1:100 | - |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 2 publications |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Horse, Human, Mouse, Non-human primate, Rat |
| Published Species | Horse, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 74T2 |
| Immunogen | Recombinant protein derived from the N-terminus of human Caspase 3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533540 |
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce large and small subunits, which dimerize to form the active enzyme.
Caspase 3 cleaves and activates caspases 6, 7, and 9, and is itself processed by caspases 8, 9, and 10. It is the predominant caspase involved in cleavage of amyloid-beta 4A precursor protein, linked to neuronal death in Alzheimer’s disease. Alternative splicing results in two transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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