Thermo Fisher Scientific RNF20 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence. Antigen range: 78-211. Recombinant protein control fragment (Product #RP-109730). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RNF20,uniProtId:Q5VTR2-1,ncbiNodeId:9606,antigenRange:78-211,antigenLength:975,antigenImageFileName:PA5-144748_RNF20_Q5VTR2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-144748_RNF20_Q5VTR2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3092174

Product Specific Information

Immunogen sequence: KLERRQATDD ASLLIVNRYW SQFDENIRII LKRYDLEQGL GDLLTERKAL VVPEPEPDSD SNQERKDDRE RGEGQEPAFS FLATLASSSS EEMESQLQER VESSRRAVSQ IVTVYDKLQE KVELLSRKLN SGDN

Antigen sequence identity: Mouse 98%; Rat 99%

Target Information

RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.