Applied Biosystems™

TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to자세히 알아보기

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.

TaqMan™ PreAmp Master Mix lets you:

• Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays
• Analyze up to 100 gene expression targets with minimal hands-on time

Preserves Equilibrium of Targets Without Preamplification Bias
In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.

Easy Workflow
With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler.

사양

검출 방법Primer-probe

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

중합효소AmpliTaq Gold DNA Polymerase

용도(장비)7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System

패시브 참조 염료No ROX

제품라인TaqMan™

제품 유형PreAmp Master Mix

수량40 reactions

충분40 Reactions

용도(애플리케이션)Pre-amplification

핫 스타트Built-In Hot Start

반응 수40 Reactions

샘플 종류cDNA

부피1 mL

Unit SizeEach