Invitrogen™

Premo Autophagy Assays with Sensor LC3B, p62, or Tandem Sensor RFP-GFP-LC3B

Detect cell autophagy with our Autophagy Assay Kits, which offer chimeric sensors containing fluorescent proteins GFP and RFP, and autophagy-associated proteins p62 and LC3B.

Easily monitor various stages of cellular autophagy with Premo Autophagy assays and sensors, which are based on LC3B- or p62-linked fluorescent protein chimeras, or the RFP-GFP-LC3B tandem sensor. Premo Autophagy assay kits offer the selectivity of Premo Autophagy sensors, which are based on chimeras of autophagy-associated proteins LC3B or p62, and green (GFP) and/or red fluorescent protein (RFP), with the transduction efficiency of BacMam technology. Transduction or even co-transduction can be performed in a wide variety of cells, including neurons and neural stem cells (NSCs) using a simple protocol that involves adding the BacMam 2.0 chimeric reagent to cells, incubating overnight, and then visualizing transduction using standard GFP (green fluorescent protein) and/or RFP (red fluorescent protein) settings.

사양

설명Premo Autophagy Sensor LC3B-GFP (BacMam 2.0)

수량1 kit

형식Slide

키트 구성품LC3B-FP, a control BacMam LC3B (G120A)-RFP, and chloroquine diphosphate

제품 유형Autophagy Sensor

호환 셀Mammalian Cells

여기/방출488/520

색상Green

배송 조건Wet Ice

제품라인Premo

검출 방법Fluorescence

용도(장비)Confocal Microscope

라벨 또는 염료LC3B-GFP

라벨 유형Other Label(s) or Dye(s)

보관 요구 사항Store at 2°C to 6°C, protected from light. Do Not Freeze.

Unit SizeEach