Thermo Fisher Scientific N-cadherin Monoclonal Antibody (3B9)
N-cadherin 특이적 Mouse Monoclonal Antibody로, P- 및 E-cadherin과 교차 반응하지 않음. WB, IHC, ICC/IF 등 다양한 응용 가능. Recombinant chicken N-Cadherin 도메인으로 면역화. 0.5 mg/mL 농도의 액상 형태로, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 65 publications |
| Immunohistochemistry (IHC) | – | View 48 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–5 µg/mL | View 26 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 8 publications |
| Immunocytochemistry (ICC/IF) | 1–3 µg/mL | View 28 publications |
| Flow Cytometry (Flow) | – | View 2 publications |
| Immunoprecipitation (IP) | 3–5 µg | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 18 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Pig, Rat |
| Published Species | Avian, Dog, Hamster, Horse, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Tag, Zebrafish |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3B9 |
| Immunogen | Recombinant domain corresponding to the intracellular domain of chicken N-Cadherin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2313779 |
Product Specific Information
This antibody is specific for N-cadherin and does not cross-react with other cadherin family members including P- and E-cadherins.
For IHC with this antibody, use formalin-fixed, paraffin-embedded tissue and heat-induced epitope retrieval.
Target Information
N-cadherin is a 140 kDa transmembrane protein mediating calcium-dependent intercellular adhesion. Cadherins regulate morphogenetic movements during development and control cell surface adhesion through homotypic interactions.
N-cadherin’s function depends on its association with the actin-cytoskeleton via catenin proteins, with stability regulated by phosphorylation of beta-catenin.
It is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
The protein plays essential roles during gastrulation and in establishing left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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