Thermo Fisher Scientific Phospho-AS160 (Thr642) Recombinant Rabbit Monoclonal Antibody (10H18L7)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

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Product Specifications

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

10H18L7

Immunogen

Peptide with the sequence RRRAH[pT]FSHPPS corresponding to amino acids 637-648 of AS160

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

RRID

AB_2532329

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

AS160 (TBC1D4) is a GTPase-activiting protein for RAB2A, RAB8A, RAB10, and RAB14. The isoform 2 version of this protein promotes the insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane. This movement increases glucose uptake. Mutations associated with the gene cause Diabetes mellitus, non-insulin-dependent, 5 (NIDDM5).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.