Thermo Fisher Scientific Apolipoprotein J Polyclonal Antibody
Apolipoprotein J에 특이적인 Thermo Fisher Scientific의 goat polyclonal antibody로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 인간 및 생쥐 반응성. 연구용으로만 사용 가능.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Apolipoprotein J Polyclonal Antibody
Thermo Fisher Scientific Apolipoprotein J Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ELISA | 0.1–0.4 µg/mL (Detection), 0.2–0.8 µg/mL (Capture) |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Pig |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Clusterin (Glu22–Glu448) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608081 |
Product Specific Information
- In sandwich ELISAs, approximately 5% cross-reactivity with recombinant human Clusterin is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Isoform 1 functions as an extracellular chaperone that prevents aggregation of nonnative proteins and stress-induced aggregation of plasma proteins. It inhibits amyloid fibril formation by APP, APOC2, B2M, CALCA, CSN3, SNCA, and aggregation-prone LYZ variants (in vitro).
Does not require ATP and maintains partially unfolded proteins for refolding by other chaperones such as HSPA8/HSC70.
Secreted isoform protects cells against apoptosis and complement cytolysis. Intracellular isoforms interact with ubiquitin and SCF E3 ligase complexes, promoting proteasomal degradation of target proteins and modulating NF-κB transcriptional activity. Nuclear isoforms promote apoptosis, while mitochondrial isoforms suppress BAX-dependent cytochrome c release and inhibit apoptosis. Plays a role in regulating cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 없음)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.