Thermo Fisher Scientific EDA2R Monoclonal Antibody (2A10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

2A10

Immunogen

EDA2R (AAH34919.1, 2 a.a. approximately 135 a.a) partial recombinant protein with mouse IgG2a-Fc tag. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EDA2R,uniProtId:Q9HAV5-1,ncbiNodeId:9606,antigenRange:2-135,antigenLength:297,antigenImageFileName:H00060401-M11_EDA2R_Q9HAV5-1_House_mouse.svg,antigenImageFileNamePDP:H00060401-M11_EDA2R_Q9HAV5-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: DCQENEYWDQ WGRCVTCQRC GPGQELSKDC GYGEGGDAYC TACPPRRYKS SWGHHRCQSC ITCAVINRVQ KVNCTATSNA VCGDCLPRFY RKTRIGGLQD QECIPCTKQT PTSEVQCAFQ LSLVEADAPT VPPQ

Target Information

X-linked ectodysplasin-A2 receptor (XEDAR) is a recently isolated member of the tumor necrosis factor receptor family that is highly expressed during embryonic development and binds to ectodysplatin-A2 (EDA-A2). Two predominantly expressed isoforms, XEDAR-s and XEDAR-L, differ by only a 21-amino region at the juxtamembrane region of the cytoplasmic domain. Neither isoform possesses a death domain and both have been shown to act mainly through TRAF3 and TRAF6 to activate the NF-kappa-B and JNK pathways. Cells transfected with XEDAR and treated with EDA-A2 cause the assembly of a secondary complex containing FADD, caspase-8 and caspase-10, leading to the activation caspase-8 and caspase-3, and finally apoptosis. The EDA-A2-induced apoptosis is dependent on caspase-9 activation, as various pharmacological and genetic inhibitors of caspase-8 blocked apoptosis following EDA-A2 treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.