Thermo Fisher Scientific Thrombopoietin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Neutralization (Neu)

0.1-0.3 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Thrombopoietin/Tpo and Sf 21-derived recombinant mouse Thrombopoietin/Tpo

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608847

Product Specific Information

This antibody neutralizes the biological activity of recombinant mouse Thrombopoietin/Tpo. This antibody will also neutralize the activity of recombinant human (rh) Thrombopoietin/Tpo, although 25 times the amount of Ig is required. In direct ELISAs less than 15% cross-reactivity with rhTpo is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.