Thermo Fisher Scientific Pericentrin/Kendrin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunocytochemistry (ICC/IF)

1:1,000-1:5,000

-

Immunoprecipitation (IP)

2-10 µg/mg lysate

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 175 and 225 of human Pericentrin/Kendrin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Pericentrin,uniProtId:O95613-1,ncbiNodeId:9606,antigenRange:175-225,antigenLength:3336,antigenImageFileName:A301-348A-M_Pericentrin_O95613-1_Rabbit.svg,antigenImageFileNamePDP:A301-348A-M_Pericentrin_O95613-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For ICC, formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

Target Information

This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.