Thermo Fisher Scientific SFTPB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:6,000

-

Product Specifications

Species Reactivity

Bovine

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

SP-B purified from Infasurf.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

20 mg/mL

Purification

IgY fraction

Storage buffer

PBS, pH 7.2

Contains

0.075% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2185367

Product Specific Information

SA1-72016 detects Surfactant Protein B in human, mouse, and rat samples.

SA1-72016 has been successfully used in western blot procedures.

The SA1-72016 immunogen is from Chicken immunized with SP-B purified from Infasurf.

Target Information

Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kDa) and SP-D (43kDa) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kDa) and SP-C (4kDa) are non-collagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B is glycosylated in the Golgi apparatus and undergoes carboxy- and amino-terminal proteolysis by a cathepsin D-like protease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.