Takara SapphireAmp fast PCR—hot-start master mix

SapphireAmp Fast PCR Master Mix is designed for fast, streamlined PCR, and is, therefore, ideal for applications such as colony PCR. It contains a hot-start PCR enzyme, optimized buffer, dNTPs, along with a blue gel-loading dye and density reagent, in a convenient 2X premix format.

Reactions are assembled by simply mixing primers and DNA template with SapphireAmp Fast PCR Master Mix. For rapid and convenient screening, the amplification reactions can be loaded directly onto an agarose gel immediately after amplification without purification, or digested directly with restriction enzymes.

For _E. coli_-based colony PCR, inserts up to ~5 kb in length are easily screened; colony PCR can be performed in about an hour.

For human genomic DNA, amplification of 2-kb targets can be completed in one hour; amplifications of up to 6 kb are possible.