Invitrogen™

pcDNA™6.2/V5-PL-DEST Mammalian Expression Vector

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-pL-DEST vector offers자세히 알아보기

The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-pL-DEST vector offers the following key features:

•Promoterless version of the pcDNA™6.2⁄V5-DEST vector (cat. no. 12489027)
•_att_R sites for Gateway™ cloning
•Compatible with MultiSite Gateway™ Pro kits (e.g. cat. no. 12537100)
•C-terminal V5 tag for easy detection
•Blasticidin resistance gene for efficient stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Gateway™ Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have _att_R sites for recombination with any _att_L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.

Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.

사양

배달 유형Transfection

용도(장비)MultiSite Gateway™ Pro kits (e.g. cat. no. 12537-100)

프로모터None (Promoterless)

제품 유형Mammalian Expression Vector

발현 시스템Mammalian

단백질 태그 위치(유전자에 대해)C-terminal

단백질 태그V5 Epitope Tag

클로닝 방법Gateway

수량6 μg

벡터pcDNA, pDEST, MultiSite Gateway Vectors

제품라인Gateway™, pcDNA™

용도(애플리케이션)Clone your own Promoter, Multi-Gene Expression

Unit SizeEach