
Thermo Fisher Scientific RAD18 Monoclonal Antibody (2G8C10)
Thermo Fisher Scientific RAD18 Monoclonal Antibody (2G8C10)은 인간 RAD18 단백질을 인식하는 Mouse IgG1 항체로, Western blot, Flow cytometry, ELISA 등에 사용됩니다. 고순도 Protein G 정제, 액상 형태이며 연구용으로 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G8C10 |
| Immunogen | Purified recombinant fragment of human RAD18 (AA: 332–430) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090926 |
Target Information
Human Rad18 (hRad18) is a homologue of S. pombe Rad18. In yeast, Rad6 and Rad18 play crucial roles in post-replication repair, filling gaps in daughter strands during replication of damaged DNA. The Rad6–Rad18 interaction is critical in the early steps of lesion bypass. In vivo, Rad18 forms a tight complex with Rad6 via a conserved ring-finger motif, binding to damaged sites through single-stranded DNA binding. This leads to degradation of stalled replication components via the ubiquitin-conjugating enzyme activity of Rad6.
Human RAD18 maps to chromosome 3p24–25, a region often deleted in lung, breast, ovary, and testis cancers. The gene encodes a 495 amino acid protein (~56 kDa) and is ubiquitously expressed across human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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