Thermo Fisher Scientific OTUB1 Monoclonal Antibody (OTI1E3), TrueMAB
OTUB1 단백질을 표적으로 하는 Mouse IgG1 단일클론 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Lyophilized 형태로 제공되며 PBS buffer와 trehalose 포함. 인체 유래 OTUB1 전체 길이 단백질을 면역원으로 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E3 |
| Immunogen | Full length human recombinant protein of human OTUB1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
OTUB1 is a member of the OTU superfamily of predicted cysteine proteases.
This protein acts as a highly specific ubiquitin iso-peptidase, cleaving ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates.
It interacts with other ubiquitin proteases and E3 ubiquitin ligases that inhibit cytokine gene transcription in the immune system.
OTUB1 is proposed to function in specific ubiquitin-dependent pathways, possibly by editing polyubiquitin chain growth.
Alternative splicing produces multiple transcript variants.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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