Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), NovaFluor Blue 690

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor belonging to the immunoglobulin gene superfamily. The N-terminal amino acid sequence (aa1–107) shows high homology to Ig kappa light chains, while the carboxyl-terminal region is proline-rich, forming a stalk-like structure projecting the Ig domain.

CD7 is expressed on most immature and mature T lymphocytes, T cell leukemias, natural killer cells, a small subset of normal B cells, and malignant B cells. Cross-linking of surface CD7 enhances T cell and NK cell activity, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 directly associates with phosphoinositol 3′-kinase, and ligation induces D-3 phosphoinositide production and tyrosine phosphorylation.

A subpopulation of human CD34(+) CD38(-) cord blood cells expressing CD45RA, HLA-DR, and high CD7 levels has lymphoid progenitor capacity, generating B cells, NK cells, and dendritic cells but not myeloid or erythroid cells. CD7(+) phenotype distinguishes primitive lymphoid progenitors from pluripotent stem cells. CD7 may cooperate with CD28 in Treg function; deficiency in both reduces Treg numbers and activity.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.25 µg)/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining. Add 5 µL per sample prior to combining with cells.
• For single-color controls, use 5 µL CellBlox per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, enabling engineered fluorescent signatures optimized for spillover and spread management.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

CD7 (gp40, Leu9) is a 40 kDa immunoglobulin superfamily member expressed on immature and mature T lymphocytes, T cell leukemias, NK cells, and subsets of B cells. It modulates immune cell activity through calcium signaling, adhesion molecule expression, and cytokine secretion. CD7 expression is a key marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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